METHOD FOR PREPARING L-SUBCULTURES OF BRUCELLA ABORTUS, STRAIN I-206 Russian patent published in 2005 - IPC

FIELD: microbiology, immunology.

SUBSTANCE: proposed invention characterizes a method for preparing L-subcultures of Brucella abortus, strain I-206. Method involves passage of brucella of the strain Brucella abortus I-206 in nutrient medium comprising a liver infusion, normal equine serum, agar-agar. Sodium chloride, magnesium sulfate, sucrose and glycerol with addition of bicillinum-3 to the nutrient medium in increasing concentrations wherein these components are taken in the following ratio, wt.-%: agar-agar, 1.2; sodium chloride, 0.1; magnesium sulfate, 0.05; sucrose, 10; glycerol, 1.0; normal equine serum, 10; liver infusion, the balance, wherein bicillinum-3 is added to the nutrient medium in increasing concentrations from 10 to 9000 U/ml of medium. Method provides preparing L-subcultures of brucella in the final concentration of bicillinum-3 up to 2000 U/ml of medium wherein these microorganisms in a single intravenous administration in rabbits cause the production of specific noncomplete antibodies detected by the Kumbs reaction at titer value 1:400. At the final concentration of bicillinum-3 9000 U/ml of medium L-subcultures of brucella are prepared that in a single intravenous administration in rabbits cause the production of specific agglutinins detecting in the agglutination reaction at titer value 1:1600. Invention can be used in immunology in aim for preparing highly active and highly specific brucella L-forms serum.

EFFECT: improved preparing method.

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