FIELD: biotechnologies.
SUBSTANCE: method of obtaining of brucellous L-antigen is performed as follows. The strain Brucella abortus 19 is cultivated in the L-form in the dense nutrient medium prepared on the basis of meat-peptonic hepatic glucose-glyceric agar (1.3-1.5%) with adding of 10-15% normal horse Serum at the temperature 20-22°C within 2-3 days. The culture, obtained in the L-form, is emulsified with 0.5% phenolised normal saline solution, inactivated at the temperature 85-90°C within 60 minutes. The suspension is centrifuged at 3000-5000 rpm within 15-20 minutes and the concentration of brucellas is established at the level 50-60 billion m.k. The obtained antigen is titrated, standardised by specificity and activity. It is used for agglutination test in serum diagnostics of brucellosis.
EFFECT: invention allows to minimise the time of antigen preparation and to increase the yield of bacterial mass.
4 tbl, 3 ex
Title | Year | Author | Number |
---|---|---|---|
METHOD FOR PRODUCING BRUCELLOUS L-ANTIGEN | 2011 |
|
RU2486916C2 |
METHOD OF PRODUCTION OF BRUCELLA L-ANTIGEN | 2013 |
|
RU2533811C1 |
METHOD FOR PREPARING L-SUBCULTURES OF BRUCELLA ABORTUS, STRAIN I-206 | 2003 |
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RU2263142C2 |
STRAIN OF BACTERIUM BRUCELLA ABORTUS USED FOR PREPARING DIAGNOSTIC AND PROPHYLACTIC BIOPREPARATIONS AGAINST BRUCELLOSIS IN ANIMALS | 1999 |
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RU2149183C1 |
METHOD FOR PRODUCTION OF DIAGNOSIS SERUM AGAINST L-FORM BRUCELLA | 2003 |
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RU2268748C2 |
VACCINE AGAINST CATTLE BRUCELLOSIS | 1997 |
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RU2108110C1 |
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|
RU2133470C1 |
STRAIN BRUCELLA ABORTUS USED FOR PREPARING DIAGNOSTIC AND PROPHYLACTIC BIOPREPARATIONS AGAINST BRUCELLOSIS IN ANIMALS | 1997 |
|
RU2130068C1 |
WAY OF RECEPTION OF BRUCELLOUS ANTIGEN FROM STRAIN BRUCELLA ABORTUS 19 FOR MANUFACTURING OF UNIFORM BRUCELLOUS ANTIGEN FOR AA (AGGLUTINATION ASSAY), CFR (COMPLEMENT FIXATION REACTION) AND LCFR (LONG-TERM COMPLEMENT FIXATION REACTION), BRUCELLOUS ANTIGEN FOR ROSE BENGAL ASSAY (RBA) AND BRUCELLOUS ANTIGEN FOR RING TEST (RT) WITH MILK, WAY OF MANUFACTURING OF BRUCELLOUS DIAGNOSTIC SERUM AND DIAGNOSTIC SETS | 2008 |
|
RU2361610C1 |
METHOD FOR MAKING BRUCELLOSIS DIAGNOSTIC SERUM | 2013 |
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RU2549434C2 |
Authors
Dates
2015-01-27—Published
2013-08-27—Filed