METHOD OF OBTAINING OF BRUCELLOUS L-ANTIGEN Russian patent published in 2015 - IPC C12N1/00 A61K39/02 

Abstract RU 2539827 C1

FIELD: biotechnologies.

SUBSTANCE: method of obtaining of brucellous L-antigen is performed as follows. The strain Brucella abortus 19 is cultivated in the L-form in the dense nutrient medium prepared on the basis of meat-peptonic hepatic glucose-glyceric agar (1.3-1.5%) with adding of 10-15% normal horse Serum at the temperature 20-22°C within 2-3 days. The culture, obtained in the L-form, is emulsified with 0.5% phenolised normal saline solution, inactivated at the temperature 85-90°C within 60 minutes. The suspension is centrifuged at 3000-5000 rpm within 15-20 minutes and the concentration of brucellas is established at the level 50-60 billion m.k. The obtained antigen is titrated, standardised by specificity and activity. It is used for agglutination test in serum diagnostics of brucellosis.

EFFECT: invention allows to minimise the time of antigen preparation and to increase the yield of bacterial mass.

4 tbl, 3 ex

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RU 2 539 827 C1

Authors

Gordienko Ljubov' Nikolaevna

Popova Tamara Gavrilovna

Kulikova Elena Vladimirovna

Gajdutskaja Galina Mikhajlovna

Elantseva Natal'Ja Borisovna

Dates

2015-01-27Published

2013-08-27Filed