RECOMBINANT PLASMID DNA pHI03, CODING HYBRID PROTEIN WITH HUMAN PROINSULIN, CELL Escherichia coli, TRANSFORMED BY RECOMBINANT PLASMID DNA pHI03, AND STRAIN OF BACTERIA Escherichia coli JM109/pHI03-PRODUCENT OF HYBRID PROTEIN-PRECURSOR OF HUMAN INSULIN Russian patent published in 2014 - IPC C12N15/00 C12N15/17 C12N1/21 

Abstract RU 2515061 C1

FIELD: biotechnologies.

SUBSTANCE: invention represents a recombinant plasmid DNA pHI03, guiding synthesis of hybrid human protein with human proinsulin, containing DNA that codes hybrid protein with human proinsulin with size of 134 a.o. with amino acid sequence shown in fig.2, containing amino acid sequences of a leader peptide, in the form of an N-end fragment of human gamma-interferon connected to human proinsulin by peptide linker. On the basis of the recombinant plasmid pHI03 a strain Escherichia coli JM109/pHI03 is produced - producer of hybrid protein with proinsulin, registered in the Federal State Unitary Enterprise GosNIIgenetikie VKPM under No.V-11409.

EFFECT: usage of proposed inventions makes it possible to simplify technology of human insulin extraction and to increase its yield.

4 cl, 2 dwg, 2 ex

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RU 2 515 061 C1

Authors

Shmatchenko Vadim Vasil'Evich

Dates

2014-05-10Published

2013-04-29Filed