FIELD: biotechnologies.
SUBSTANCE: invention represents a recombinant plasmid DNA pHI03, guiding synthesis of hybrid human protein with human proinsulin, containing DNA that codes hybrid protein with human proinsulin with size of 134 a.o. with amino acid sequence shown in fig.2, containing amino acid sequences of a leader peptide, in the form of an N-end fragment of human gamma-interferon connected to human proinsulin by peptide linker. On the basis of the recombinant plasmid pHI03 a strain Escherichia coli JM109/pHI03 is produced - producer of hybrid protein with proinsulin, registered in the Federal State Unitary Enterprise GosNIIgenetikie VKPM under No.V-11409.
EFFECT: usage of proposed inventions makes it possible to simplify technology of human insulin extraction and to increase its yield.
4 cl, 2 dwg, 2 ex
| Title |
Year |
Author |
Number |
| RECOMBINANT PLASMID DNA pHILP07 ENCODING HYBRID PROTEIN WITH HUMAN PROINSULIN Lispro, CELL Escherichia coli TRANSFORMED BY RECOMBINANT PLASMID DNA pHILP07, AND STRAIN OF BACTERIA Escherichia coli JM109/pHILP07 - PRODUCER OF HYBRID PROTEIN WITH HUMAN PROINSULIN Lispro |
2013 |
- Shmatchenko Vadim Vasil'Evich
|
RU2514480C1 |
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2013 |
- Shmatchenko Vadim Vasil'Evich
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|
RU2515115C1 |
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2013 |
- Shmatchenko Vadim Vasil'Evich
- Sadgjan Armen Sergeevich
|
RU2514578C1 |
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2006 |
- Shmatchenko Vadim Vasil'Evich
- Shmatchenko Natal'Ja Anatol'Evna
- Bajdus' Aleksandr Nikolaevich
- Kuptsov Vasilij Nikolaevich
- Nozdrin Viktor Nikolaevich
- Stepanov Aleksej Vjacheslavovich
|
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2004 |
- Shmatchenko V.V.
- Piskareva O.A.
- Shmatchenko N.A.
- Bajdus' A.N.
- Stepanov A.V.
|
RU2263147C1 |
| RECOMBINANT PLASMID DNA PF265 CODING HYBRID POLYPEPTIDE CONTAINING HUMAN PROINSULIN, AND BACTERIAL STRAIN ESCHERICHIA COLI - PRODUCER OF HYBRID POLYPEPTIDE CONTAINING HUMAN PROINSULIN |
2019 |
- Latypov Vitaliy Felixovich
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|
RU2728611C1 |
| STRAIN OF BACTERIA Escherichia coli JM109/pHINS21 - PRODUCER OF HYBRID PROTEIN WITH HUMAN PROINSULIN AND METHOD FOR PRODUCTION OF HUMAN PROINSULIN |
2007 |
- Rodionov Petr Ivanovich
- Rodionov Petr Petrovich
- Shmatchenko Vadim Vasil'Evich
- Stepanov Aleksej Vjacheslavovich
- Bajdus' Aleksandr Nikolaevich
- Shmatchenko Natal'Ja Anatol'Evna
- Gorkun Taras Alekseevich
|
RU2376368C2 |
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2019 |
- Latypov Vitaliy Felixovich
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- Robustova Svetlana Eduardovna
- Khomutova Oksana Sergeevna
- Rodionov Petr Petrovich
|
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| RECOMBINANT PLASMID DNA PF644 CODING HYBRID POLYPEPTIDE CONTAINING PROINSULIN GLARGINE, AND BACTERIAL STRAIN ESCHERICHIA COLI - PRODUCER OF HYBRID POLYPEPTIDE CONTAINING PROINSULIN GLARGINE |
2019 |
- Latypov Vitaliy Felixovich
- Kornakov Igor Aleksandrovich
- Robustova Svetlana Eduardovna
- Khomutova Oksana Sergeevna
- Rodionov Petr Petrovich
|
RU2729381C1 |
| RECOMBINANT PLASMID DNA PF646 CODING HYBRID POLYPEPTIDE CONTAINING PROINSULIN ASPART, AND STRAIN OF BACTERIA ESCHERICHIA COLI - PRODUCER OF HYBRID POLYPEPTIDE CONTAINING PROINSULIN ASPART |
2019 |
- Latypov Vitaliy Felixovich
- Kornakov Igor Aleksandrovich
- Robustova Svetlana Eduardovna
- Khomutova Oksana Sergeevna
- Rodionov Petr Petrovich
|
RU2729353C1 |
