FIELD: bioassay methods.
SUBSTANCE: invention, in particular, relates to a reagent system for detecting substances. Signal generation system for detecting substances in a sample contains at least one first and second electron transfer agent and redox indicator. Electron transfer agents utilized are those of protein and nonprotein nature, for example phenazine compound and diaphorase. System further contains at least one of following compounds: enzyme cofactor, enzyme manifesting oxidative activity relative to tested substance, e.g. corresponding dehydrogenase. Proposed systems, reagent compositions, test strips, and kits find use in detection of a large number of substances in a sample such as a biological sample, e.g. blood or blood fraction.
EFFECT: increased reaction rate of signal generation system and reduced cost.
8 cl, 1 dwg, 3 tbl
