FIELD: biotechnology, in particular production of bioactive supplements useful in treatment and prophylaxis of various gastrointestinal diseases in any kind of agriculture animals and poultry.
SUBSTANCE: claimed method includes introducing of lactobacterium cultures Lactobacillus acidophilus and Lactobacillus delbrueskii subsp. Bulgaricus in equal ratio in broth containing lightened milky whey, yeast extract, manganese sulfate, and potassium phosphate. Lactobacterium biomass is grown by deep cultivation process at 44±1°C. During cultivation medium pH is adjusted and double neutralization is carried out. After 2-3 h form cultivation starting Lactobacillus lactic subsp. lactic III Defatted milk, 40 % glucose solution are introduced into broth followed by further cultivation. Before cultivation finishing sodium citrate is introduced and mixture is cooled. Cooled biomass is centrifuged ad separated. Concentrated biomass is blended with sucrose/gelatin protective medium and potassium iodide, pre-packed in penicillin flacks, and freeze dehydrated.
EFFECT: inexpensive harmless and convenient preparation having high antagonistic properties against pathogenic microflora and increase concentration of healthy cells.
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