METHOD FOR QUANTITATIVE DETECTING PROTEIN IN BIOLOGICAL LIQUIDS Russian patent published in 2006 - IPC G01N33/68 

FIELD: medicine, clinico-diagnostic practice.

SUBSTANCE: the present innovation deals with carrying out analyses for the quantitative protein content in biological liquids (either in urine or spinal liquid). The method deals with mixing solution of reagents and a sample of biological liquid, measuring optic density of the solution of developed complex and calculation of protein content by calibrating graph or equation of calibrating graph. Solution of reagents includes pyrogallol red, sodium molybdate, succinic acid, sodium acetate, low-atomic alcohol and a stabilizer. As a low-atomic alcohol the solution contains ethanol, and as a stabilizer - nonionogenic SAS. Mixing the sample o biological liquid and that of reagents takes place at the ratio of 1:(10-50), and measuring optic density should be carried out photometrically at wave length or several wave lengths ranged 600-700 nm. The innovation provides widened range of linearity and decreased expenses of reagents.

EFFECT: higher sensitivity and accuracy of detection.

2 cl, 1 dwg