STRAIN "VNIIZZH" OF CATTLE CORONAVIRUS FOR PREPARING DIAGNOSTIC, VACCINE AND CURATIVE PREPARATIONS Russian patent published in 2006 - IPC C12N7/00 A61K39/215 A61P31/12 

FIELD: veterinary virology and biotechnology.

SUBSTANCE: invention proposes the strain that possesses high antigenic and immunogenic activity. The strain is deposited in the Collection of microorganism strain FGU VGNKI at the registration name "VNIIZZH-DEP". The coronavirus strain is reproduced in transplanted cell cultures of calf kidney (MDVK, Taurus), calve coronary vessel cells and in heterologous culture of simian cells (Vero). After 3-4 days of incubation at 37°C virus is accumulated in the amount up to 10⁸ particels/cm³ by data of electronic microscopy. The strain shows ability for reproducing in the same accumulation degree in cell culture MDVK at 34°C. The strain possesses high biological, antigenic and immunogenic activity and retains native immunobiological properties after inactivation and can be used in preparing highly sensitive and specific diagnostic, highly immunogenic, harmless and areactogenic vaccine and curative preparations.

EFFECT: valuable properties of strain, improved preparing method.

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