FIELD: biotechnology, biochemistry, amino acids.
SUBSTANCE: invention relates to a method for producing L-amino acids, for example, L-isoleucine and L-leucine prepared by culturing a microorganisms in medium and able to produce L-amino acid. This microorganism carries the avtA gene encoding enzyme alanine-valine transaminase (transaminase C) and possesses the enhanced activity of this enzyme as compared with it's the parent strain. Accumulated L-isoleucine and L-leucine are isolated from the cultural fluid. Invention provides preparing L-isoleucine and L-leucine with high effectiveness degree by diminishing the amount of amino acid as a by-side product that results to complications in the process of purification of indicated L-amino acids.
EFFECT: improved producing method of amino acids.
8 cl, 1 dwg, 2 tbl, 3 ex
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