METHOD OF STREPTOCOCCUS ANATOXIN PREPARING Russian patent published in 2002 - IPC

FIELD: biotechnology, medicine, microbiology. SUBSTANCE: method involves streptococci culturing in nutrient medium containing the following components, g/l of distilled water: citric acid, 4.9-5.0; potassium hydrogen phosphate, 4.9-5.0; sodium chloride, 1.9-2.0; magnesium sulfate, 4.9-5.0; iron sulfate, 0.04-0.05; asparagine, 0.9-1.0; glycocol, 0.9-1.0 and glycerol, 3.0-3.1. Sterilization of the grown biomass is carried out in an autoclave at 0.8-0.9 atm for 35-40 min followed by detoxification and separation of biomass by filtration. Anatoxin shows the good expressed curative effectiveness in different clinical forms of respiratory diseases in calves. EFFECT: improved method of preparing. 1 tbl, 1 ex