FIELD: medicine.
SUBSTANCE: there is reconstructed recombinant plasmid DNA pGD-10 coding a chimeric protein construct DsD-sp-β-GAL containing a dextrane-binding domain DBD from Leuconostoc mesenteroides microorganism which is genetically combined through a glycine-serine spacer with thermostable beta-galactosidase from thermophilic Thermoanaerobacter ethanolicus microorganism. The Escherichia coli DH5α/pGD-10 strain producing the recombinant protein construct DsD-sp-β-GAL is produced by transforming the cells of parental Escherichia coli DH5α strain by recombinant plasmid DNA pGD-10.
EFFECT: invention allows producing protein DsD-sp-β-GAL exhibiting enzymatic activity of thermostable beta-galactosidase and affine connectable with dextrane.
3 cl, 4 dwg
| Title | Year | Author | Number |
|---|---|---|---|
| RECOMBINANT PLASMID DNA pGD-14 CONTAINING HYBRID GENE, INCLUDING NUCLEOTIDE SEQUENCE OF DEXTRAN-BINDING DOMAIN OF BETACOCCI, JOINED WITH HUMAN INTERFERON-GAMMA GENE THROUGH ACID-LABILE SPACER, DETERMINING BIOSYNTHESIS OF CHIMERIC PROTEIN STRUCTURE | 2011 |
|
RU2470072C1 |
| RECOMBINANT PLASMID pESAT6-CFP10-DBD, RECOMBINANT STRAIN Escherichia coli M15 [pREP4, pESAT6-CFP10-DBD], METHOD FOR PREPARING, IMMOBILISING, CONCENTRATING AND PURIFYING RECOMBINANT PROTEIN ESAT6-CFP10-DBD ON DEXTRAN, RECOMBINANT PROTEIN ESAT6-CFP10-DBD AND IMMUNOGENIC COMPOSITION CONTAINING PROTEIN ESAT6-CFP10-DBD | 2013 |
|
RU2539026C1 |
| METHOD OF PRODUCTION OF IMMUNOGENIC COMPOSITION BASED ON FUSION PROTEIN pESAT6-DBD AND DEXTRAN, RECOMBINANT PLASMID pESAT6-DBD, STRAIN OF Escherichia coli, CHIMERIC PROTEIN ESAT6-DBD AND THEIR APPLICATION | 2013 |
|
RU2520737C1 |
| METHOD FOR OBTAINING IMMUNOGENIC COMPOSITION BASED ON Ag85A-DBD HYBRID PROTEIN AND DEXTRANE; pAg85A-DBD RECOMBINANT PLASMIDE; Escherichia coli [pREP4, pAg85A-DBD] STRAIN; Ag85A-DBD CHIMERIC PROTEIN | 2013 |
|
RU2520078C1 |
| METHOD FOR OBTAINING IMMUNOGENIC COMPOSITION BASED ON CFP10-DBD HYBRID PROTEIN AND DEXTRANE; pCFP10-DBD RECOMBINANT PLASMIDE; Escherichia coli [pREP4, pCFP10-DBD] STRAIN; CFP10-DBD CHIMERIC PROTEIN AND THEIR APPLICATION | 2013 |
|
RU2546875C1 |
| RECOMBINANT PROTEIN LACspCBD POSSESSING BETA-GALCTOSIDASE ACTIVITY AND ABILITY FOR SPONTANEOUS BINDING WITH CELLULOSE-CONTAINING SORBENTS, RECOMBINANT PLASMID DNA ENCODING SYNTHESIS OF RECOMBINANT PROTEIN LACspCBD, STRAIN Escherichia coli M15 [pREP4, pLACspCBD] AS PRODUCER OF RECOMBINANT PROTEIN LACspCBD, METHOD FOR PREPARING IMMOBILIZED RECOMBINANT PROTEIN LACspCBD ON CELLULOSE AND METHOD FOR ENZYMATIC CLEAVAGE OF LACTOSE | 2004 |
|
RU2278160C2 |
| METHOD OF PREPARING IMMUNOGENIC COMPOSITION BASED ON THREE FUSION PROTEINS OF SHELL OF TICK-BORNE ENCEPHALITIS VIRUS DEFINING BELONGING TO SIBERIAN (DBD2-D3S), EUROPEAN (DBD2-D3E) AND FAR EAST (DBD2-D3D) VIRUS SUBTYPES; RECOMBINANT PLASMIDS pDBD2-D3S, pDBD2-D3E AND pDBD2-D3D; PRODUCER STRAINS Escherichia coli M15 [pREP4]; CHIMERIC PROTEINS AND THEIR APPLICATION | 2014 |
|
RU2560588C1 |
| CHIMERIC PROTEIN FOR NON-VIRAL TRANSGENOSIS, INCLUDING DNA-BINDING DOMAIN SSBTne AND SIGNAL OF NUCLEAR LOCALISATION OF VirD2 | 2008 |
|
RU2376317C1 |
| METHOD FOR Trib-2mut RECOMBINANT ELASTROMERIAL DOMAIN, PRODUCTION, GENE-ENGINEERING pGDTrib2mut CONSTRUCTION, DETERMINING Trib-2mut BIOSYNTHESIS IN E.coli CELLS, E.coli M15/pGDTrib2mut PRODUCING STRAIN AND METHOD FOR POLYMER MATERIAL OBTAINING BASED ON THIS PROTEIN | 2015 |
|
RU2631004C2 |
| IMMUNOGENIC COMPOSITION BASED ON RECOMBINANT PSEUDO ADENOVIRAL PARTICLES, AS WELL AS BASED ON PROTEIN ANTIGENS AND A METHOD FOR PRODUCING AN IMMUNOGENIC COMPOSITION | 2018 |
|
RU2691302C1 |
