FIELD: gene engineering, in particular production of transgenic potato with Colorado beetle resistance and biological and food safety.
SUBSTANCE: recombinant polynucleotide sequence of general formula X-A, wherein X is part of nucleotide sequence of introduced genetic construct representing in SEQ ID N 1; A is nucleotide sequence of flanking site of genomic DNA of genus Nevskiy potato, representing in SEQ ID N 2 is constructed; plant cell producing of cryIIIa protein and containing recombinant polynucleotide sequence, as well as potato transgenic plant and vegetative generation thereof with Colorado beetle resistance are obtained. Polynucleotide sequence is used in identification of plant cell producing of cryIIIa protein, plant and vegetative generation.
EFFECT: new potato genus with Colorado beetle resistance.
6 cl, 6 dwg, 7 tbl, 7 ex
| Title | Year | Author | Number |
|---|---|---|---|
| RECOMBINANT POLYNUCLEOTIDE SEQUENCE CHARACTERIZING UNIQUE TRANSFORMATION ACT BETWEEN GENETIC CONSTRUCT INCLUDING cryIIIa GENE, AND GENOMIC DNA OF GENUS ELIZAVETA POTATO, USES THEREOF, TRANSGENIC PLANT AND GENERATION THEREOF | 2004 |
|
RU2286385C2 |
| RECOMBINANT POLYNUCLEOTIDE SEQUENCE MARKING UNIQUE TRANSFORMATION ACTION BETWEEN GENETIC MAKER INCLUDING GENE cryIIIA AND GENOMIC DNA OF LUGOVSKOY POTATO; ITS APPLICATION; CELL, TRANSGENIC PLANT, AND ITS PROGENY CONTAINING THIS SEQUENCE | 2007 |
|
RU2337529C1 |
| INSECT-RESISTANT AND HERBICIDE TOLERANT BREEDING STACK OF SOYBEAN EVENT PDAB9582.814.19.1 AND PDAB4468.04.16.1 | 2012 |
|
RU2627161C2 |
| NUCLEIC-ACID SEQUENCE FOR TRANSGENIC SOYBEAN TRANSFORMANT DBN9004 DETECTION IN A BIOLOGICAL SAMPLE, A KIT CONTAINING SUCH A SEQUENCE, AND A METHOD FOR SUCH DETECTION | 2017 |
|
RU2743397C2 |
| PLANT TRANSFORMANTS OF PV-ZMIR13 (MON863) MAIZE AND COMPOSITIONS AND METHODS OF IDENTIFYING THEM | 2003 |
|
RU2352638C2 |
| LINES OF TRANSGENIC SOYA, GENETIC EVENT 8264,42,32,1, RESISTANT TO HERBICIDES WITH PACKAGED GENES BASED THEREON, AND THEIR DETECTION | 2012 |
|
RU2636021C2 |
| CORN PLANT DBN9858 RESISTANT TO HERBICIDE, AND NUCLEOTIDE SEQUENCE AND METHOD FOR DETECTION THEREOF | 2016 |
|
RU2712730C2 |
| ELITE E-GM3 EVENT AND METHODS AND KITS FOR IDENTIFICATION OF SUCH EVENT IN BIOLOGICAL SAMPLES | 2010 |
|
RU2639530C2 |
| MAIZE PLANT DBN9936 AND METHOD OF USING IN DETECTION NUCLEIC ACID SEQUENCE THEREOF | 2016 |
|
RU2707527C2 |
| INSECT RESISTANT AND HERBICIDE TOLERANT SOYBEAN EVENT 9582.814.19.1 | 2012 |
|
RU2628099C2 |
