FIELD: biotechnology, vaccines.
SUBSTANCE: method involves infection of transplantable cells VNK-21 with virus as measured for 0.01-0.001 MMLD 50/cell, multiplied for 36-70 h in suspended state and inoculated on microcarriers in the dose (1.2-2.5) x 105 cells in 0.3-0.6 ml of nutrient medium per each 1 cm2 of microcarriers. Culturing cells on microcarriers is carried out in bioreactor for 5-6 days and virus-containing cultural liquid is collected every day. Prepared vaccine possesses the high immunogenic activity and method for its preparing shows the high output.
EFFECT: improved preparing method of vaccine.
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