FIELD: biotechnology; virology.
SUBSTANCE: disclosed is a method of culturing highly pathogenic influenza A virus. Method involves the following steps: a) cell culture propagation in a culture fluid-free medium, b) cell culture infection with influenza A virus, c) incubating an influenza virus infected cell culture under conditions which allow an increase in the influenza virus population, wherein cell culture used is a culture of yeast mitochondria Saccharomyces cerevisiae SI79-5A (VKPM Y-100).
EFFECT: method allows avoiding the use of mammalian organ cell culture.
3 cl, 7 dwg, 1 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| RECOMBINANT STRAIN OF YEASTS Schizosaccharomyces pombe - PRODUCENT OF LACTIC ACID | 2013 |
|
RU2539092C1 |
| METHOD OF REPLICATION OF INFLUENZA VIRUS IN CULTURE | 2007 |
|
RU2491339C2 |
| METHOD OF PREPARING HCV PROTEINS USEFUL FOR USING IN VACCINE OR IMMUNE ANALYSIS, ASIALOGLYCOPROTEIN (VARIANTS), COMPOSITION FOR USING IN VACCINE OR IN IMMUNE ANALYSIS (VARIANTS), METHOD OF ASIALOGLYCOPROTEIN PURIFICATION AND A METHOD OF HCV CONTENT DECREASE OR ELIMINATION | 1991 |
|
RU2123528C1 |
| METHOD FOR OBTAINING PROTEIN DUE TO USING TRANSLATION SYSTEM OF MITOCHONDRIA | 1997 |
|
RU2201961C2 |
| HEPATITIS C VIRUS (HCV) ASIALOGLYCOPROTEIN, IMMUNOGENIC COMPOSITION, METHOD OF INDUCTION OF IMMUNE RESPONSE, METHOD OF IMMUNE ASSAY | 1991 |
|
RU2175657C2 |
| ANTIVIRAL COMPOUND OF MULTIPLE ACTION, ITS COMPOSITION AND METHOD OF TREATING VIRAL DISEASES | 2012 |
|
RU2597150C2 |
| METHOD TO PRODUCE PROTEIN E7-HSP70 AND YEAST STRAIN Saccharomyces cerevisiae FOR ITS REALISATION | 2012 |
|
RU2489481C1 |
| METHOD FOR INITIAL ISOLATION OF INFLUENZA VIRUS A STRAIN, STRAIN VIRUS A/DUCK/NOVOSIBIRSK/56/05 H5N1 FOR PREPARATION FOR DIAGNOSIS, PROPHYLAXIS AND THERAPEUTICAL AGENTS, AND FOR EVALUATION OF ANTIVIRAL ACTIVITY OF VARIOUS COMPOUNDS | 2005 |
|
RU2309983C2 |
| SCHIZOSACCHAROMYCES POMBE STRAIN - LACTIC ACID PRODUCER | 2016 |
|
RU2650669C1 |
| METHOD FOR VIRUS REPLICATION IN AVIAN EMBRYO STEM CELLS | 2006 |
|
RU2457253C2 |
