METHOD FOR DETERMINING PHAGOCYTIC ACTIVITY OF NEUTROPHILS BY MICROBIOLOGICAL APPROACH Russian patent published in 2020 - IPC G01N33/48 C12Q1/02 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, particularly to clinical immunology. Disclosed is a method of determining the phagocytic activity of neutrophils, in which the initial concentration of the solution of Saccharomyces cerevisiae is 0.00125 %; for experimental and control groups initial concentration of mononuclear cells - 2⋅10⁶ cells/ml; for the test and control groups in a round-bottom polystyrene plate or in eppendorf tubes, mixture of 100 mcl of Saccharomyces cerevisiae solution and 100 mcl of mononuclear solution is made; plate or test tubes are incubated with anaerobic culture apparatus content at temperature of 36 ± 1 °C for 60 minutes; in 60 minutes from each well of the plate or from each test tube, contents of 100 mcl are inoculated into separate Petri dishes, followed by incubation in anaerobic culture apparatus at 26 ± 2 °C for 18 hours; after 18 hours in each Petri dish, Saccharomyces cerevisiae colonies are counted and the NPA is compared in the test and control groups, wherein the immunosuppression state reduces the functional activity of the neutrophils, which is manifested in the form of a greater number of grown colonies of Saccharomyces cerevisiae in the test group compared to the control, and in the immunostimulation state, a decrease in the number of the grown Saccharomyces cerevisiae colonies in the test group as compared to the control is observed.

EFFECT: invention provides a wider range of methods for assessing nonspecific immunity.

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