FIELD: medicine.
SUBSTANCE: method involves incubating transplants for preventing calcinosis occurrence in salt solution containing no calcium and phosphate ions but calcium chelators in the amount enough for initiating donor cells death and preventing calcinosis centers formation. Ethylene diamine tetraacetic acid is applied as chelator in 0.2-2.0% concentration or sodium citrate in 1-2.5% concentration. Digitonin is applied in concentration reaching 0.1% for increasing effectiveness and donor cells death. Incubation is carried out at salt solution pH being within the range of 5.0-7.5 and temperature from 10 to 37°C during 2-4 days.
EFFECT: enhanced effectiveness in preventing calcinosis and causing donor cells death; high transplant biocompatibility.
2 cl, 3 dwg
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