FIELD: medicine, cardiovascular surgery.
SUBSTANCE: the method includes incubation in solution with agents causing lethality in donor's cells, incubation in nutritive medium to wash agents off. Incubation should be carried out at 37 C during the day in 0.02%-ethylene diamine tetraacetic acid solution or ethylene diamine tetrasodium acetate in Hanks' solution, gentamicin 400 mg/l, flucanasol 20 mg/l followed by incubation at 37 C during the day in minimum calcium-free Eagle's nutritive medium at addition of 0.02%-ethylene diamine tetraacetic acid or ethylene diamine tetrasodium acetate. The present innovation enables to achieve decreased tissue capacity and calcification in combination with decreased immune reaction to an implant.
EFFECT: higher efficiency.
2 dwg, 3 ex, 1 tbl
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