METHOD FOR IMPROVING BIOCOMPATIBILITY OF VALVE AND VASCULAR GRAFTS Russian patent published in 2013 - IPC A61L27/36 A61F2/24 A61F2/06 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, particularly to methods for improving the biocompatibility of valve and vascular grafts ensured by reducing the immunogenicity and preventing the calcification. The method for improving the biocompatibility of valve and vascular grafts consists in pre-incubation of the tissue grafts in normal saline containing 0.9% sodium chloride, EDTA (0.5-2 mM), organic buffer HEPES (5-20 mM) at pH 7.0 for 4-6 hours, incubation in normal saline containing 0.9% sodium chloride, HEPES 5-20 mM (pH 7.8), 1% sodium deoxycholate for 30-48 h; the tissue grafts are washed from sodium deoxycholate in a solution containing 0.9% sodium chloride, HEPES 5-20 mM (pH 7.8) and 20% ethanol for 8 days with the medium replaced for the fresh one every day and washed from ethanol for one day.

EFFECT: method for improving the biocompatibility enables preventing the graft calcification, reducing their cell toxicity and the immune response to the tissue grafts.

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