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2 294 374

(13)

(51)

IPC

C12Q1/68(2006-01-01)

C12N15/31(2006-01-01)

(21) (22)

Application

2004125139/13, 2004-08-03

(24)

Start date

2004-08-03

(22)

Actual filing date

2004-08-03

(45)

Published

2007-02-27

(72)

Inventor

Semenikhin Vladimir IvanovichJurik Sof'Ja AntonovnaDudareva Ekaterina Viktorovna

(73)

Holder

Gosudarstvennoe Nauchnoe Uchrezhdenie Institut Ehksperimental'Noj Veterinarii Sibiri I Dal'Nego Vostoka So Raskhn Iehvsidv So Raskhn)

METHOD FOR DETERMINATION OF PATHOGENIC SUBSPECIES OF FUSOBACTERIUM NECROPHORUM SUBSPECIES NECROPHORUM IN SAMPLE USING POLYMERASE CHAIN REACTION Russian patent published in 2007 - IPC C12Q1/68 C12N15/31

Abstract RU 2294374 C2

FIELD: veterinary microbiology, molecular biology, bio-industry.

SUBSTANCE: invention proposes a method for determination of pathogenic subspecies of Fusobacterium necrophorum subspecies necrophorum in a sample. Method involves synthesis of oligonucleotide primers, synthesis of complementary DNA on genome DNA matrix and amplification of synthesized complementary DNA by the polymerase chain reaction method. Method involves using oligonucleotide primers № 180 and № 190, and analysis of polymerase chain reaction (PCR) is carried out in 1.0% agarose gel. Method can be used in veterinary.

EFFECT: improved determination method.

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RU 2 294 374 C2

Authors

Semenikhin Vladimir Ivanovich

Jurik Sof'Ja Antonovna

Dudareva Ekaterina Viktorovna

Dates

2007-02-27—Published

2004-08-03—Filed