METHOD OF PREPARING BACTERIAL AGENT RHODER FOR CLEANING SOILS, LANDS, OIL SLIMES, FRESH AND MINERALIZED WATERS TO REMOVE PETROLEUM AND PETROLEUM PRODUCT POLLUTION Russian patent published in 2007 - IPC B09C1/10 C02F3/34 C12N1/20 

FIELD: biotechnological techniques in environmental pollution control.

SUBSTANCE: invention relates to preparing oil destruction agents suitable for cleaning oil-polluted soils and water. In particular, bacterial agent Rhoder is based on strains Rhodococcus rubber BKM As-1513D and Rhodococcus erythropolis BKM Ac-1514 D and prepared in several steps. Preparation procedure is distinguished by that, in the first step, most active clones of strain R forms belonging to oil destruction agent are selected and, in the last step, common deep culturing of two strains is performed, the two strains having been preliminarily grown in inoculators on specially selected nutrient media under physiologically optimal culturing conditions in large scale. In the maximum biomass production step, biomass is grown on nutrient medium containing glucose as only carbon source and autolyzed yeast or fish hydrolyzate as source of nitrogen and other organics. Preparation according to invention enables preparing liquid or dry agent with number of active hydrocarbon-oxidizing cells 1011-1012 cell/mL and 1010-1011 cell/mL, respectively, which eliminates need in further performing preliminary activation of dry agent.

EFFECT: enhanced oil oxidizing activity of bacterial agent and shortened preparation time without loss in efficiency and qualitative characteristics.

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