FIELD: biotechnology, pisciculture.
SUBSTANCE: the innovation deals with preparing nutritive media, inoculation material and obtaining sensitin out of the strains Vibrio anguillarum N2 and VUR-19 followed by sensitization of ram's erythrocytes. Moreover, it is necessary to apply 20%-suspension of formalinized erythrocytes upon phosphate-buffer solution at pH being 7.0-7.4. Sensitization should be carried out upon water bath in two stages. Erythrocytes and sensitin should be taken at the ratio of 1:0.3-1:0.35.
EFFECT: higher efficiency.
2 ex
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