METHOD OF MANUFACTURING ERYTHROCYTE DIAGNOSTIC KIT FOR DIAGNOSTICS OF PULLOROSE TYPHUS IN BIRDS Russian patent published in 1997 - IPC

Abstract RU 2085949 C1

FIELD: biotechnology. SUBSTANCE: method of preparing pullor erythrocyte antigen includes following operations: resuspending of surfactant with added soda or sodium hydroxide followed by extraction at 93-96 C for 60 min, centrifugation and filtration to produce antigen sensitin extract, layering of antigen sensitin onto formulated erythrocytes, clarification of sensitized sheep erythrocytes through precipitation technique, and dilution thereof to 10% concentration. EFFECT: accelerated process (from 15 to 5 dys), excluded use of acetic acid and ethanol, and improved quality of preparation. 3 cl, 1 tbl

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RU 2 085 949 C1

Authors

Skichko Nikolaj Danilovich

Mel'Nik Nikolaj Vasil'Evich

Zenov Nikolaj Ivanovich

Zheltov Viktor Viktorovich

Erokhin Vladimir Ivanovich

Chukaeva Elena Nikolaevna

Dates

1997-07-27Published

1996-04-10Filed