FIELD: biotechnology. SUBSTANCE: method of preparing pullor erythrocyte antigen includes following operations: resuspending of surfactant with added soda or sodium hydroxide followed by extraction at 93-96 C for 60 min, centrifugation and filtration to produce antigen sensitin extract, layering of antigen sensitin onto formulated erythrocytes, clarification of sensitized sheep erythrocytes through precipitation technique, and dilution thereof to 10% concentration. EFFECT: accelerated process (from 15 to 5 dys), excluded use of acetic acid and ethanol, and improved quality of preparation. 3 cl, 1 tbl
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Authors
Dates
1997-07-27—Published
1996-04-10—Filed