FIELD: biotechnology, transplantation, in particular, isolation of hematopoietic stem cells.
SUBSTANCE: Lin-negative or weak positive cells derived from bone marrow are cultivated in presence of macrophage colony-stimulating factor (M-CSF) at concentration of approximately 5-20 ng/ml together with cytokine composition including stem cell factor (SCF), interleukin-11 (IL-11) ligand such as fms-like tyrosine kinase (FLT) or thrombocytopoietin (TPO), wherein cytokines are used in the next weight ratio: 1-4 M-CSF:20 SCF:20 IL-11:20 (FLT or TPO).
EFFECT: effective method for targeted propagation of hematopoietic stem cells.
4 cl, 3 dwg, 1 ex
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Year |
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Number |
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|
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|
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2012 |
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