FIELD: medicine.
SUBSTANCE: human haemopoetic stem cells are separated either from bone marrow or mobilised peripheral blood, or blood separated from umbilical vein. The fractionation procedure is used to prepare in Ficoll gradient from total cell mass the suspension of mononuclear cells wherefrom enriched suspension of cells CD133+ and CD34+ is separated. The prepared suspension is cultivated in the environment Dulbecco M (IMDM) with insulin and transferrin, as well as the stem cell growth factor (SCF), tyrosine kinase ligand 3 of fetal liver (Flt3L), thrombopoietin (TPO) and ionomycin (Ca2+ ionophore). After 7-10 days of cultivation, the grown cell suspension contains 80-92% of CD34+ cells.
EFFECT: invention allows for short-term cultivation of higher number of low-differentiated haemopoetic stem cells to be used in therapeutic purposes.
8 dwg, 4 tbl, 11 ex
