FIELD: biothechnology, medicine.
SUBSTANCE: invention relates to new mutant forms of multisubstrate deoxyribonucleoside kinase of Drosophila melanogaster (Dm-dNK) having altered kinetic properties compared with wild type enzyme. Expression of said forms in cell makes it possible to reduce of cell mortal dose (LD100) at least one nucleoside analog used as prodrug and selected from group: AZT, aga-A, aga-C, ddC, ddA, and 2 CdA. Described are nucleotide sequences encoding Dm-dNK variants, vector constructs including the same, method for production of enzyme mutant forms and uses of new proteins and polynucleotides in pharmaceutical compositions.
EFFECT: cytotoxic and antiviral agents of increased effectiveness and selectivity.
7 cl, 5 dwg, 2 tbl, 4 ex
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