FIELD: biotechnology.
SUBSTANCE: invention proposes a method based on transformation of inclusion bodies to a soluble form using organic denaturating reagents and chromatography methods. In carrying out chromatography inorganic, alkaline and salt-containing eluents are chosen. After termination of purification recombinant variants of allergen Bet v 1 are prepared in form that can be used in medicinal aims directly. The advantage of method involves minimal treatment of a sample, using pharmacologically compatible substances and effective removing endotoxins in process of treatment of the end protein solution.
EFFECT: improved method of isolation and purification.
2 tbl, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR PREPARING BIRCH POLLEN HYPOALLERGIC BASIC ALLERGEN r Bet v 1 | 2003 |
|
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