FIELD: chemistry.
SUBSTANCE: invention relates to field of biotechnology, namely to method of obtaining purified recombinant GDF-5-like protein. Method includes destruction of E.coli cells by homogeniser of high pressure at pressure of destruction from 800 to 900 bar. Inclusion bodies are separated from destructed cells E.coli by centrifugation. Separated inclusion bodies are processed with denaturate buffer, which contains carbamide and L- arginine, for solubilisation. Solubilised monomer of recombinant GDF-5-like protein is purified by centrifugation and chromatography.
EFFECT: invention makes it possible to obtain purified recombinant GDF-5-like protein with high output.
7 cl, 10 dwg, 2 tbl, 5 ex
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