METHOD FOR SUPPRESSING THE REPRODUCTION OF CATTLE INFECTIOUS RHINOTRACHEITIS VIRUS IN CELL CULTURE Russian patent published in 2007 - IPC A61K31/41 

FIELD: veterinary virology.

SUBSTANCE: the innovation deals with inoculation of finite cell line MDBK due to microanalysis in 96-cell culture trays. Then cell culture should be supplemented with cattle infectious rhinotracheitis virus at the dosage of 1-10 TCD_50/cell to incubate cells with virus for about 1.5-2 h. Then one should add antiviral preparation at intoxic dosage of 50±1 mcg/ml. One should evaluate antiviral activity of ribavirin in 72 h of incubating cells and virus at 37±0.2C under atmospheric conditions of 5±0.2% CO₂. The innovation enables to prevent toxic action of the preparation upon the cells due to decreasing its dosage and provides the chance to carry out therapy in earlier terms.

EFFECT: higher efficiency.

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