CELL LINE OF CALF KIDNEY Bos taurus RBT (Rene Bos Taurus) FOR REPRODUCTION OF ANIMAL VIRUSES Russian patent published in 2013 - IPC C12N5/07 A61K39/12 

FIELD: biotechnologies.

SUBSTANCE: permanent cell line of calf kidney Bos taurus RBT, deposited in 2010 into the Russian Collection of Cell Cultures (V) (Russian Academy of Agricultural Sciences, agricultural animals) under the No.74. The RBT line is produced from primarily trypsinized kidney cells of a 2-month calf in process of 65 serial passages in a single layer in the medium of 0.4 GLA on Earl with 10% foetal serum of cattle. Forms a population of epithelial cells, which is homogeneous by morphology, with considerable polymorphism in karyotype. At the same time population of cells with 51 chromosomes makes 32%, in contrast to normal karyotype Bos taurus (60 chromosomes), in RBT there are 9 metacentric and 1 submetacentric chromosomes, the number of polyploids is not more than 2%.

EFFECT: RBT line has high sensitivity to a corona virus, a virus of infectious rhinotracheitis, a plague virus and virus diarrhoea, and also high proliferative activity and ability for growth in rotary vessels.

4 dwg, 5 tbl, 4 ex