FIELD: molecular biology.
SUBSTANCE: invention provides a method to determine hydrophobic ligands of lipid-carrying proteins and to find out lipid-binding and lipid-carrying activities of proteins, which would clarify lipid-protein interactions in vivo. Method comprises preparation of donor and acceptor phases, which are then juxtaposed them, while separating them by porous barrier, after solution of test protein in SET buffer is passed and then acceptor part is extracted by tertiary mixture of equal parts of chloroform, methanol, and diethyl ether to recover protein solution.
EFFECT: simplified procedure.
3 dwg, 3 ex
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