FIELD: biotechnology.
SUBSTANCE: disclosed is expression vector containing ColE1 replication system wherein homology of RNAI and RNAII of ColE1 replication initiating site with free tRNA is modified by one or more mutation in encoding region of RNAI or RNAII gene. Said mutations lead to substitution of one or more bases in loop 1, 2, 3 of RNAI or RNAII. Also described are bacterial cell transformed by said vector and method for production of desired protein from abovementioned bacterial cell. Claimed method includes cell culturing, protein isolation and purification thereof. Increased homology ratio of RNAI and RNAII with free tRNA causes increased amount of plasmida copies. Decreased homology ratio of RNAI and RNAII with free tRNA causes reduced metabolite load in cell that is highly for recombinant protein production.
EFFECT: improved method for controlling of plasmida copy amount.
12 cl, 1 dwg, 7 tbl, 2 ex
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