RING DNA MOLECULE WITH CONDITION-DEPENDENT REPLICON ORIGIN, METHOD FOR PRODUCTION THEREOF AND USES IN GENE THERAPY Russian patent published in 2007 - IPC C12N1/21 C12N15/00 C12N15/64 C12R1/01

Abstract RU 2307870 C2

FIELD: biotechnology.

SUBSTANCE: disclosed is procariotic recombinant host cell containing heterologous protein of replication activation, which activated condition-dependent replicon origin, and extrachromosomal DNA molecule, containing heterologous therapeutic gene and condition-dependent replicon origin. Also disclosed are method for producing of plasmid containing heterologous therapeutic gene and condition-dependent replicon origin, plasmid representing suicide vector for gene transferring, containing heterologous therapeutic gene and condition-dependent replicon origin.

EFFECT: method for avoiding of non-controlled overexpression of therapeutic gene and resistance genes.

44 cl, 41 dwg, 10 tbl, 14 ex

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RU 2 307 870 C2

Authors

Subrie Fab'Enn

Dates

2007-10-10—Published

2003-10-14—Filed