FIELD: biochemistry.
SUBSTANCE: invention relates to recombinant antibody (AB) to EpCAM, containing amino acid sequence defining carcass region of Ig L-chain and certain amino acid residues of sequence represented in SEQ ID NO:5 (such and following sequences are as defined in description). Described is variant of recombinant AB to EpCAM, containing amino acid sequence defining carcass region of Ig H-chain, representing certain amino acid residues of sequence of SEQ ID NO:5. Another variant of recombinant AB is AB to EpCAM, containing in L-chain 1-106 amino acid residues of SEQ ID NO:9, and in H-chain amino acid residues of SEQ ID NO:18. Also disclosed are expression vectors wherein each vector contains DNA nucleotide sequence encoding respective amino acid sequence of each AB variant.
EFFECT: recombinant antibodies with decreased immunogenicity in contrast to starting mouse antibodies to EpCAM.
1q5 cl, 6 tbl, 5 ex
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