HUMANISATION OF RABBIT ANTIBODIES USING UNIVERSAL ANTIBODY SCAFFOLD Russian patent published in 2015 - IPC C07K16/00 C12N15/13 C12N15/63 

FIELD: biotechnologies.

SUBSTANCE: acceptor scaffold of variable region of heavy chain of human antibody or humanised antibody for transplantation of CDR from Lagomorpha antibody is presented where the scaffold contains threonine (T) in the position 24; alanine (A) or glycine (G) in the position 56; threonine (T) or asparagine (N) in the position 84 and leucine (L) or valine (V) in the position 89 (AHo numbering). The named acceptor antibody scaffold for transplantation of Lagomorpha CDR is offered. Besides, the present invention describes the released nucleinic acid coding the acceptor antibody scaffold; the expression vector containing the named nucleinic acid; and the host cell containing the named expression vector for production of the acceptor antibody scaffold.

EFFECT: invention allows to obtain the acceptor scaffold of heavy chain of antibody suitable for change of hyper variable regions of Lagomorpha antibodies.

10 cl, 6 dwg, 5 tbl, 5 ex