METHOD FOR DETERMINING GESTATION AGE OF FETUSES AND NEWBORNS Russian patent published in 2007 - IPC A61B10/00 G01N33/48 

FIELD: forensic medicine.

SUBSTANCE: method involves taking samples of fetal liver and spleen and carrying out their morphometric examination. Linear shrinkage coefficients are determined in tissues under study from relative linear sizes reduction observed on standard organ fragments before fixation and after having prepared histological preparations. Hemopoietic activity of parenchyma is determined on histological slices of 4-5 mcm thickness by counting mean quantity of nuclear fragment profiles of myeloid cells infiltrating lobal parenchyma in test vision field of histological slice having area of 2.95*10^-2 mm², and mean lymph nodes diameter in mcm and mean density of lymph nodes distribution by counting mean quantity of lymph nodes nuclear fragment profiles in spleen in test vision field of histological slice having area of 1.76*10^-2 mm². Then, real values of the parameters are determined with shrinkage taken into account from formulas: x₁=n_ba/k³ _L, x₂=d*k_L, x₃=n_ln/k² _L, where x₁, x₂, x₃ are the hemopoietic liver activity indices, diameter and density of spleen lymph nodes distribution with shrinkage taken into account, n_ba, d, n_ln are hemopoietic liver activity indices, diameter and density of spleen lymph nodes distribution with shrinkage taken into account, respectively, k_L is the linear shrinkage coefficient equal to 1.203 for liver tissue and 1.161 for spleen tissue. Point estimate of gestation age in weeks is determined as y=38.118-0.427x₁+4.374*10^-3 x₁ ² -1.5*10^-5 x₁ ³ + 2.43*10^-2x₂ - 4.29 lgx₃, where 38.118,-0.427, 4.374*10^-3 ,-1.5*10^-5 , 2.43*10^-2,-4.29 are regression coefficients. Integral estimates of fetuses and newborns gestation age are determined from nomogram on calculated point estimate value.

EFFECT: high accuracy in determining gestation age in examining fragmented fetus cadavers and fetuses with severe developmental delay syndrome.

1 dwg