FIELD: analytical methods in food industry and biotechnology.
SUBSTANCE: method envisages electrophoretic separation of components to be determined with the aid of consecutively forcing test sample into two ends of capillary preliminarily filled with working electrolyte. Sample is further affected by electric field followed by registration of components using conductometric detector to produce electrophoregram, from which individual components are qualitatively and/or quantitatively identified. Water-based working electrolyte contains 25-35 mmole histidine, 1.5-2.5 mmole Crown ether, and 0.02-0.50 mmole Triton X-100. According to invention, test sample is forced into capillary at pressure 20-70 mbar for 10-30 sec and working voltage of electric field is 28-30 kV. Individual components are cations, amines, inorganic and organic acid anions. Method may be, in particular, applied in alcoholic drink and soft drink industries.
EFFECT: increased reliability of determination and universality of analysis conditions due to enabled use of the same conditions when proceeding with both aqueous and water-alcohol media.
Title | Year | Author | Number |
---|---|---|---|
WORKING ELECTROLYTE FOR DETERMINING ION COMPOSITION OF LIQUID MEDIA USING CAPILLARY ELECTROPHORESIS | 2006 |
|
RU2315299C1 |
METHOD OF DETERMINING VIOLATE ADMIXTURES OF NITROGEN BASES IN INTERMEDIATE PRODUCTS OF ALCOHOL PRODUCTION | 2006 |
|
RU2320973C1 |
METHOD OF NON-ALCOHOLIC AND ALCOHOLIC BEVERAGE ANALYSIS FOR ORGANIC ACIDS WITH CAPILLARY ELECTROPHORESIS METHOD | 2007 |
|
RU2350938C1 |
METHOD OF QUANTITATIVE DETERMINATION OF ORGANIC ACIDS (ACETIC, MALIC, LACTIC) AND CARBOHYDRATES (MALTOSE, GLUCOSE, FRUCTOSE) IN SEMIPRODUCTS OF ALCOHOL PRODUCTION USING THE METHOD OF HIGH-EFFICIENT LIQUID CHROMATOGRAPHY | 2017 |
|
RU2653570C1 |
METHOD OF DETERMINING TOTAL NITROGEN BY CAPILLARY ELECTROPHORESIS | 2013 |
|
RU2554799C2 |
DIAGNOSTIC TECHNIQUE FOR URINARY STONE DISEASE | 2011 |
|
RU2484468C2 |
METHOD OF COMBINED DETERMINATION OF Cu(II), Pb(II), Fe(III) AND Bi(III) IONS BY METHOD OF CAPILLARY ZONE ELECTROPHORESIS | 2012 |
|
RU2535009C2 |
PROCEDURE DETECTING ANION SURFACE-ACTIVE SUBSTANCES IN WATER-CARRYING MEDIUM | 2001 |
|
RU2199107C2 |
METHOD OF DETERMINING TOTAL PHOSPHORUS BY CAPILLARY ELECTROPHORESIS | 2012 |
|
RU2499989C1 |
METHOD FOR QUANTITATIVE DETERMINATION OF PHLORIDZIN IN APPLE FRUITS AND LEAVES USING CAPILLARY ELECTROPHORESIS | 2022 |
|
RU2813773C1 |
Authors
Dates
2007-12-27—Published
2006-09-20—Filed