METHOD FOR QUANTITATIVE DETERMINATION OF PHLORIDZIN IN APPLE FRUITS AND LEAVES USING CAPILLARY ELECTROPHORESIS Russian patent published in 2024 - IPC A01G7/00 G01N27/26 

FIELD: crop production.

SUBSTANCE: method involves multiple dilution of a sample of apple juice to a phloridzin content of no more than 200 mg/dm³, or a sample from leaves, which is previously ground in a mortar with a 10% aqueous-alcohol solution in a ratio of 1:10, centrifugation and analysis. In this case, a capillary electrophoresis system is used with a quartz capillary having an effective length of 0.5 m, an internal diameter of 75 mcm and an aqueous leading electrolyte containing 3 g/dm³ imidazole, 3 g/dm³ sodium tetraborate decahydrate, 0.2 g/dm³ sodium sulphate, with positive voltage polarity and detection wavelength – 254 nm.

EFFECT: rapid and reliable quantitative determination of phloridzin by capillary electrophoresis using non-toxic and accessible chemicals for analysis in the absence of additional purification of extracts or apple juice before analysis.

1 cl, 2 dwg, 1 tbl, 4 ex