METHOD FOR PREPARING ANTIMICROBIAL PEPTIDES WITH DECREASED HEMOLYTIC ACTIVITY Russian patent published in 2008 - IPC A61K38/17 C07K14/435 A61P31/04 A61P31/10 

FIELD: chemical-pharmaceutical industry, biochemistry, chemistry of peptides.

SUBSTANCE: invention relates to biologically active latarcin peptides possessing antimicrobial effect that can be used in biotechnology and medicine. Method for preparing antimicrobial latarcin peptides with reduced hemolytic activity by chemical or biotechnological method involves preliminary modeling the space structure of the parent latarcin peptide with high hemolytic activity in its α-helical configuration and change of hydrophobic amino acid residues for hydrophilic residue in the synthesis process when hydrophobic amino acids Ile⁷ and Phe¹⁰ are replaced for hydrophilic amino acids Gln⁷ and Lys¹⁰. After modeling the space structure of the parent latarcin peptide in its α-helical configuration measurement of hydrophobic properties of peptide is carried out additionally and with taking into account the molecular hydrophobic potential value in each point of peptide surface and detection of hydrophobic clusters and amino acid residues localized in them followed by identification of the most effective hydrophilic amino acid residues for using in the synthesis process. Method provides preparing peptides with significantly reduced hemolytic properties and to decrease cytotoxicity of latarcin peptides.

EFFECT: improved preparing method of peptides.

3 tbl, 2 dwg, 6 ex