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(19)

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2 317 333

(13)

(51)

IPC

C12Q1/68(2006-01-01)

C12N15/40(2006-01-01)

(21) (22)

Application

2006102903/13, 2006-02-01

(24)

Start date

2006-02-01

(22)

Actual filing date

2006-02-01

(45)

Published

2008-02-20

(72)

Inventor

Seregin Sergej ViktorovichPetrov Vladimir SemenovichPetrova Irina DmitrievnaTjunnikov Georgij Ivanovich

(73)

Holder

Federal'Noe Gosudarstvennoe Uchrezhdenie Nauki Nauchnyj Tsentr Virusologii I Biotekhnologii Federal'Noj Sluzhby Po Nadzoru V Sfere Zashchity Prav Potrebitelej I Blagopoluchija Cheloveka Gnts Vb Rospotrebnadzora)

THE SET OF OLIGODEOXYRIBONUCLEOTIDE PRIMERS FOR IDENTIFYING MEASLES VIRUS IN CLINICAL SAMPLES Russian patent published in 2008 - IPC C12Q1/68 C12N15/40

Abstract RU 2317333 C2

FIELD: biotechnology, gene engineering, medicine.

SUBSTANCE: it has been elaborated the set of primers for reliable indication of genetic material of measles virus by taking into account the variations of genome structure of all known geographic viral isolates, in the territory of different Russian regions, as well. The set of oligonucleotide primers for identifying measles virus RNA contains 2 pairs of oligonucleotides that possess the activity of direct and reverse primers in reaction of reverse transcription and polymerase chain reaction (PCR) that have got the following structure: RF1:5'CGT(C,T)CTTAC(G,C)GCCCGCCCCG3'(20 n); RR2:5'GCGAGGCGCACGGGGACAGG3' (20 n); RF3:5'CCC(C,A)GAACTGGTGAGCCCCATGGG3' (24 n); RR4:5'CGCACCCCGG(C,T)GCAGTGC3' (18 n). The innovation enables to detect genetic material of measles virus in clinical samples.

EFFECT: higher reliable indication of genetic material of measles.

cl, 2 dwg, 6 ex, 2 tbl

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RU 2 317 333 C2

Authors

Seregin Sergej Viktorovich

Petrov Vladimir Semenovich

Petrova Irina Dmitrievna

Tjunnikov Georgij Ivanovich

Dates

2008-02-20—Published

2006-02-01—Filed