FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology and concerns a method for differential amplification of a fragment of the VP1 region of the enterovirus genome of enterovirus A and Enterovirus B by "semi-nested" polymerase chain reaction using oligonucleotide primers: in 1st round of PCR: 486: 5'-TGGTAICARACIAAITWYGTIGTNC-3' and 488: 5'-GTIGGRTAICCITCITARAACCAYTG-3' for DNA amplification of Enterovirus A viruses, 490: 5'-TGIGTIYTITGYRTICCITGGAT-3' and 492: 5'-GGRTTIGTIGWYTGCCA-3' for amplification of Enterovirus B virus cDNA; in 2nd round of PCR AN89: 5'-CCAGCACTGACAGCAGYNGARAYNGG-3' and EvAR - 5'-TACTGGAC-CNCCNGGNGGNRCRWACATRTA-3' for amplification of cDNA of Enterovirus A viruses, AN89: 5'-CCAGCACTGACAGCAGYNGARAYNGG-3' and EvBR: - 5'-TACTGGACCNCCNGGNGGNAYRTACATNA-3' to amplify cDNA of Enterovirus B viruses, where N is any of A, T, G or C, R is A or G, Y is C or T, W is A or T; I - inosine.
EFFECT: what is presented is a method for differential amplification of a fragment of the VP1 region of the enterovirus genome Enterovirus A and Enterovirus B.
1 cl, 4 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR AMPLIFYING THE VP1 REGION OF THE GENOME OF ENTEROVIRUS SPECIES ENTEROVIRUS B | 2021 |
|
RU2774424C1 |
| METHOD OF DETECTION AND DIFFERENTIATION OF ENTEROVIRUS RNA | 2000 |
|
RU2189396C2 |
| METHOD FOR ENTEROVIRUS GENOTYPING BY GENOME REGION 1A-1B SEQUENCING METHOD | 2019 |
|
RU2701145C1 |
| METHOD FOR IDENTIFICATION OF 5Т-HTP GENOME OF ENTEROVIRUSES OF EVI AND EVII GENE GROUP IMPLEMENTING POLYMERASE CHAIN REACTION | 2010 |
|
RU2441917C2 |
| SET OF SYNTHETIC OLIGONUCLEOTIDE PRIMERS FOR OBTAINING FULL NUCLEOTIDE SEQUENCE OF VP1 GENE OF COXSACKIE A10 ENTEROVIRUS | 2020 |
|
RU2762236C1 |
| SET OF OLIGODEOXYRIBONUCLEOTIDE PRIMERS AND FLUORESCENT-MARKED PROBES FOR ENTEROVIRUS RNA IDENTIFICATION BY REVERSE TRANSCRIPTION - POLYMERASE CHAIN REACTION WITH HYBRIDISATION FLUORESCENT DETECTION | 2011 |
|
RU2459830C1 |
| SET OF PRIMERS FOR IDENTIFICATION OF RNA STRAINS PUUMALA ORTHOHANTAVIRUS, COMMON IN THE REPUBLIC OF TATARSTAN | 2019 |
|
RU2720252C1 |
| METHOD FOR DETECTION OF INTESTINAL VIRUSES IN CLINICAL SAMPLES OF REAL-TIME MULTIPLEX PCR AND LIST OF SEQUENCIES FOR IMPLEMENTING IT | 2012 |
|
RU2506317C2 |
| SET OF OLIGODEOXYRIBONUCLEOTIDE PRIMERS AND FLUORESCENT-MARKED DNA PROBES FOR IDENTIFICATION OF RNA OF ENTEROVIRUSES, RHINOVIRUSES, VIRUSES OF HEPATITIS A AND E FROM WATER MEDIUM BY MULTIPLEX PCR METHOD | 2013 |
|
RU2542968C2 |
| SET OF OLIGODESOXYRIBONUCLEOTIDE PRIMERS AND FLUORESCENCE MARKED PROBES FOR IDENTIFYING RNA OF HUMAN RESPIRATORY SYNCYTIAL VIRUS | 2013 |
|
RU2541773C2 |
