FIELD: biotechnology and mariculture.
SUBSTANCE: invention is directed to culturing red algae in industrial scale. Method comprises taking vital material from the top portion of alga, culturing it in nutrient medium illuminated by luminescent lamps, said nutrient medium being a liquid nutrient medium and said vital material being microfragments of alga not larger than 1 mm3 in size. Cultivation is carried out over an at least 3-week period, up to 5-6 weeks, at continuous stirring and aeration, while weekly renewing nutrient medium. When it is desired to have microfragments of alga, one should take top or edge meristematic tissue or submeristematic tissue. A variant of the method is the one wherein, as vital material, cell aggregates are used, which are obtained from tissues having meristematic and submeristematic cells. To that end, top parts of alga are frozen for 10-30 min, then they are thawed out and placed for at least 1 week in nutrient medium. Further, living tissue of alga is disintegrated and thus obtained cell aggregates are placed in nutrient medium and cultured over an at least 3-week period at continuous stirring and aeration, while weekly renewing nutrient medium.
EFFECT: decreased number of alga cultivation steps and considerably simplified procedure.
5 cl, 4 dwg, 3 tbl, 7 ex
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Authors
Dates
2008-03-10—Published
2006-07-31—Filed