METHOD OF BIOLOGICALLY TESTING WATER, SOIL, AND BIOLOGICALLY ACTIVE SUBSTANCES Russian patent published in 2008 - IPC G01N33/18 G01N33/24 C12Q1/02 C12R1/865 

FIELD: environmental monitoring.

SUBSTANCE: invention relates to the area of utilizing microbiological objects for checking environmental pollution and can find use in environmental monitoring and sanitary-toxicological inspection of water, soils as well as in pharmacology when estimating safety of biologically active substances. Method consists in registration of variability of nature of colony-formation of two yeast strains Sacharomyces cerevisae exhibiting different ploideness under different medium conditions. Haploid and diploid strains are used. Toxic effect is established from lethal characteristics and mutagenicity from rise in number of mutant forms of colonies. Thereafter, induction of heritable cellular lethality is determined and remote pathology is predicted from the number of morphoses and change in the sizes of colonies.

EFFECT: improved reliability of toxico-genetic estimation due to use of sensitive haploid strains, specific reactions of haploids and diploids when affected by weak doses of mutagens.

2 dwg, 1 tbl