METHOD OF ASSESSMENT OF GENOTOXICITY OF AQUATIC ENVIRONMENTS Russian patent published in 2013 - IPC G01N33/18 C02F3/34 

FIELD: biotechnology.

SUBSTANCE: synchronised daphnia is obtained from the most prolific clone of daphnia population, except for the laboratory population. The test samples are prepared. From the obtained daphnias the even-aged, parthenogenetic ones are selected, and concurrently the exposure is carried out to the test solution (test), in water (control), in the solution of the reference toxicant (K_T ⁺) and in solution of mutagen (K_m ⁺). The physiological condition in the original daphnia generation (F₀) is recorded. The toxicity factors are determined, namely, the death rate (L₀) and the fertility rate (P₀). The physiological condition of daphnias of the second generation (F₂) is recorded and the total mutagenic activity is determined according to the formula &=f_ex/f_k where f_ex is frequency of lethal, sublethal and morphological mutations in the experiment, and a f_k is frequency of lethal, sublethal and morphological mutations in the control with the subsequent comparing its value with isoeffective concentrations of reference mutagens (K_m ⁺) and toxicants (K_T ⁺) by release of limiting nuisance value and establishing the level of genotoxic activity on the basis of the results obtained.

EFFECT: improving the accuracy of the estimate.

2 dwg, 15 tbl, 4 ex