FIELD: molecular biology, bioorganic chemistry.
SUBSTANCE: method for preparing DNA-chip involves modifying a polymer surface comprising electrophilic groups with compound of the formula: H2N-X-Si(OR)3 wherein X means propyl; R means ethyl in gaseous phase, and with a solution of compound of the formula: Y-X-Si(OR)3 wherein Y means a functional group; X means propyl; R means ethyl or methyl. Then oligonucleotide is immobilized on the modified surface of a polymer by the covalent route. Use of the invention provides decreasing the cost and enhancing effectiveness of modification of backing in production of DNA-chips.
EFFECT: improved preparing method.
11 cl, 10 dwg, 2 tbl, 14 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR PREPARING MULTIFUNCTIONAL MULTICHIP, MULTICHIP FOR SUCCESSIVE OR PARALLEL SCREENING BIOPOLYMERS, METHOD FOR ANALYSIS OF BIOPOLYMERS AND SET FOR REALIZATION OF METHOD | 2006 |
|
RU2321638C2 |
| METHOD FOR PREPARING DNA-CHIPS (VARIANTS) | 2005 |
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| SET OF DIFFERENTIATING AND SPECIFIC OLIGONUCLEOTIDES FOR IDENTIFYING DNA OF ACUTE INTESTINAL INFECTION AGENTS, METHOD OF IDENTIFYING ACUTE INTESTINAL INFECTION, MICROCHIP AND DIAGNOSTIC SYSTEM FOR CARRYING OUT METHOD | 2010 |
|
RU2509804C2 |
| USING UNMODIFIED POLYMERIC MATERIALS FOR PREPARING BIOCHIP BACKING, BIOCHIP BASED ON THEREOF AND METHOD FOR ITS PREPARING, METHOD FOR IMMOBILIZATION OF HYDROGELS ON UNMODIFIED POLYMERIC MATERIALS | 2006 |
|
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| METHOD OF PREPARING COATINGS AND FILMS UTILIZING ORGANO-INORGANIC NANOCOMPOSITE MATERIALS BASED ON FILM-FORMING ORGANIC POLYMERS WITH GRAFTED OLIGOSILOXANE CHAINS | 2005 |
|
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| TRANSFORMABLE BIOCHIP | 2007 |
|
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| COMPOSITION FOR IMMOBILIZATION OF BIOLOGICAL MACROMOLECULE IN HYDROGEL, METHOD FOR PREPARING COMPOSITION, BIOCHIP, METHOD FOR CARRYING OUT POLYMERASE CHAIN REACTION (PCR) ON BIOCHIP | 2001 |
|
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| METHOD TO PRODUCE REMOVABLE WATER-INSOLUBLE ION-EXCHANGE COATINGS ON MALDI TARGETS AND THEIR APPLICATION FOR MASS-SPECTROMETRIC ANALYSIS | 2010 |
|
RU2475543C2 |
| DIFFERENTIATING AND SPECIFIC OLIGONUCLEOTIDES FOR IDENTIFICATION OF DNA SEQUENCES OF TRANSGENE PLANTS IN FOODSTUFF, METHOD FOR IDENTIFYING TRANSGENE PRODUCTS, BIOCHIP, COMBINATION OF OLIGONUCLEOTIDES (VERSIONS) AND KIT FOR IMPLEMENTING SUCH METHOD | 2007 |
|
RU2453605C2 |
| METHOD FOR PREPARING DNA-CHIPS | 2003 |
|
RU2236467C1 |
