FIELD: biotechnology, biochemistry.
SUBSTANCE: method involves two-step preparing a complex consisting of protein to be isolated and purified with propeptide of this protein immobilized on sorbent and isolation of protein by elution from the prepared complex. At the first step of preparing a complex propeptide of protein to be isolated and purified is prepared wherein this propeptide is immobilized on sorbent. At the second step of preparing a complex the protein to be isolated and purified is contacted with its propeptide immobilized on sorbent by passing solution of this protein through a filled column. Method provides expanding assortment of agents used for isolation and purification of proteins, to simplify the process and to enhance its effectiveness based on increasing yield of the end product.
EFFECT: improved method of isolation and purification.
7 cl, 3 ex
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