FIELD: organic chemistry, biotechnology.
SUBSTANCE: invention refers to biotechnology. Method describes nucleotide sequence coding polypeptide of acetooxyacids synthetase activity (AHAS) and coded polypeptide. Sequences are given in detailed description. Polypeptide application for enzymatic production of enantiomer-concentrated amino acids with branched chain is described. Vector of acetooxyacids synthetase (AHAS) expression in cells Corynebacterium and microorganism Corynebacterium, carrying specified vector are described. Specification for primer of coding nucleotide sequence by "ПЦР" method is provided. Transfer probe applied for specified nucleotide sequences detection is described. Method of recombinant polypeptides production is discovered implying that specified nucleotide sequence is exposed to mutagenesis. Then nucleotide sequence is cloned and transferred to proper expression system. Produced polypeptides are detected and separated. There is a detailed description provided of recombinant polypeptide produced by specified method and its application for enzymatic production of enantiomer-concentrated amino acids with branched chain. This invention enables to increase production of amino acids with branched chain by zymosis.
EFFECT: increased production of amino acids with branched chain.
16 cl, 2 dwg, 3 tbl, 7 ex
