METHOD OF IMMOBILISED OSTEOGEN STROMAL BONE MARROW STEM CELL Russian patent published in 2008 - IPC A61K35/28 C12N5/08 A61F2/02 

FIELD: medicine.

SUBSTANCE: oestrogen stromal bone marrow stem cells are cultivated after been released at high purification from accompanying corpuscle of marrow aspirate without any preliminary mechanical and enzymatic processing and applications of microcarriers in monolayer culture. Porous skeleton surface is covered with double protein hydrophilic oestrogen layer by preparation incubation in collagen acetic acid solution and then in medium DMEM, containing gelatinol solution and r- glycerophosphate. Porous skeleton is biologically inert titanium nickelide metal alloy with permeable through porous structure. Suspension based on stromal bone marrow stem cells purified and grown up in monolayer cultures are applied on porous skeleton prior to be used ex tempore by diffusion deposition on hydrophilic permeable surface.

EFFECT: method application provides produced three-dimensional monoculture of oestrogen stromal bone marrow stem cells with specified amount and density of corpuscles of prolonged proliferative activity and tissue differentiation for modelled tissue structure and reparative functions of spongy bone.

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