METHOD OF ACUTE BACTERIAL ENTERIC INFECTIONS EXPRESS-DIAGNOSTICS Russian patent published in 2008 - IPC G01N33/577 

FIELD: medicine.

SUBSTANCE: invention concerns medical diagnostics. For purpose of acute bacterial enteric infections diagnostics, lymphocytic suspension is tested for acute enteric infection (AEI) agent antigen by indirect immunoperoxidase method. Centrifugated blood cells are applied on glass slide (smear), dried up at room temperature, fix in pure acetone, and then processed in 3% H₂O₂ for 20 minutes. Blood cells are incubated in blocking normal serum, incubated with poly- or monoclonal antibodies to required antigenes at t 37°C. Then they are processed with reagents of polymeric detection system: Super Enhacer™ Reagent and further with Poly-HRP Reagent. Then they are processed with 3,3-diaminobenzidine-tetrachloride (DAB). Brown granules in lymphocytes and monocytes indicate required antigen, namely infection diagnostics.

EFFECT: method application provides increased accuracy and reduced time of diagnostics of acute bacterial enteric infections.

3 dwg, 1 ex