METHOD OF POTATO MICROCLONING Russian patent published in 2008 - IPC A01H4/00 

FIELD: agriculture.

SUBSTANCE: invention agricultural biotechnologies. Healthy potato plants are cultivated in vitro by micrografting into nutrient medium containing macro- and microelements according to Murashige&Skoog formula, Fe-chelate, agar, vitamins according to White subscription, and sucrose. The regenerated plants obtained are replanted out. Additionally 3 mg/l of glycine, 1000-2000 mg/l of activated carbon and 1-2 mg/l of ascorbic acid are introduced into the nutrient medium. Original plants undergo micrografting at the apical, middle and basal parts. Apical and middle parts are vegetated in nutrient medium with sucrose content of 20 g/l at the temperature of 23-25°C in daytime and 17-18°C at night. Further regenerated potato plants are bedded. Basal part is vegetated in nutrient medium with sucrose content of 80 g/l in darkness at the temperature of 8-10°C, with further growing of potato microtubers in vitro.

EFFECT: allows improving survival rate and accelerate explants growth, increasing reproduction rate of regenerated plants and quantity of in vitro microtubers, as well as improving survival rate for regenerated plants after replanting them out.

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