METHOD OF DIFFERENTIAL DIAGNOSTICS OF ATYPICAL FOLLICULAR ADENOMA AND FOLLICULAR CANCER IN THYROID GLAND Russian patent published in 2008 - IPC A61B10/00 G01N33/569 

FIELD: medicine; oncology.

SUBSTANCE: additionally cell cytoplasm labeling indices are defined for each Bc1-2 and p53 albumen. Tissue sample is taken from the tumour cut out during operation, tumour sections for immunohistochemical analysis are prepared. At the tomour sections expression of Ki-67, Bc1-2 and p53 albumens in cell nuclei is registered by specific monoclonal antibodies in a complex with chromogen. For each albumen, cell nucleus labeling index is defined by the following procedure: average percentage of cells with nucleus coloured by the chromogens are registered over more than 30 visual fields in histological sections processed by one of the specific monoclonal antibodies. Additionally cell cytoplasm labeling indices are defined by the same procedure for Bc1-2 and p53 albumens, potential probability index for proliferation activity (PPIPA) is evaluated by the formula: PPIPA=(LInuc_Bc1-2/LInuc_p53)•LInuc_Ki-67, where LInuc_Bc1-2, LInuc_p53, LInuc_Ki-67 are cell nucleus labeling indices for each of the Bc1-2, p53 or Ki-67 albumens respectively; possible cytoplasmic atypia index (PAI) is evaluated as a ratio of cell cytoplasm labeling index of Bcl-2 albumen to cell cytoplasm labeling index for p53 albumen; tumour growth and potential malignancy index (TGPMI) is defined as a product of PAI and PPIPA; at 0≤TGPMI≤4.35 atypical follicular adenoma is indentified, and at TGPMI>4.36 follicular cancer is identified.

EFFECT: increased accuracy of post-operational differential diagnostics of follicular adenoma and follicular cancer in thyroid gland.

4 tbl, 1 ex