FLUORESCING PROTEINS AND CHROMOPROTEINS FROM KINDS HYDROZOA WHICH ARE NOT CONCERNING TO AEQUOREA, AND METHODS OF THEIR OBTAINING Russian patent published in 2008 - IPC C12N15/12 C12N15/63 C12N5/10 C12N15/62 C07K14/435 C12Q1/68 G01N33/50 G01N33/533 

FIELD: biology, gene engineering.

SUBSTANCE: invention can be used for marking of biological objects. The molecule of nucleic acid which codes the fluorescing protein chosen from fluorescing proteins of representatives of kind Phialidium sp. are both suborder Anthomedusae and fluorescing mutants of the specified proteins allocated. By means of the allocated nucleic acid are obtained cloning and expressing vectors, fluorescing protein, the protein of merge capable to fluorescence, and also the expressing cartridge. The cell and the stable cellular line, containing such express ionic cartridge, produce fluorescing fiber. The fluorescing protein, nucleic acid coding it and the express ionic genetic designs containing this nucleic acid, use in a set for marking of a biological molecule. Fluorescent protein is also used in methods of marking of a biological molecule, a cell or a cellular organella.

EFFECT: invention application allows dilating an arsenal of agents for marking of biological objects.

13 cl, 12 dwg,12 ex